Journal articles: '617.96/04 20' – Grafiati (2024)

Abstract:

Thirty six candidate cotton varieties developed by different breeders of Pakistan were tested consecutively for two years (2017 and 2018) and at seven locations of Sindh and Balochista Provinces in national coordinated varietal trials (NCVT). The trials were conducted to explore seed cotton yield potential and fiber properties against two check varieties (CIM-602 and FH-142/IUB-13). As per claim of the breeders, the samples of all varieties for both the years were sent to four designated biotechnological laboratories for conducting biochemical tests also. The results revealed highly significant differences among the varieties for both the years. During the year 2017, on an average of six location, top ten high yielding varieties recorded were GH-Haadi, Weal-AG-6, VH-189, GH-Mubarak, Weal-AG-5, MNH-1026, Badar-1(DG), FH-444, CIM-343 and TJ-Max(DG) which yielded 3434, 3407, 3342, 3255, 3251, 3248, 3185, 3154, 3134 and 3131 kg/ha seed cotton yield respectively. When the results of 2018 trial were looked at, averagely top ten high yielding varieties were GH-Haadi, ICI-2121, CRIS-613, VH-383, VH-189, NIAB-898, FH-490, Cyto-225, Tahafuz-10(DG) and GS-Ali-7 with 3526, 3356, 3306, 3139, 3101, 3091, 3084, 3074, 3060 and 3026 kg/ha of seed cotton yield respectively. However, on an average of both the years (2017 and 2018), top ten high yielding varieties were GH-Haadi, VH-189, CRIS-613, Weal-AG-6, GH-Mubarak, Badar-1(DG), ICI-2121, Weal-AG-5, FH-940 and MNH-1026 producing 3480, 3221, 3186, 3155, 3113, 3083, 3057, 3054, 3042 and 3042 kg/ha of seed cotton respectively. As regards fiber properties, (04 candidate varieties) could qualified all fiber standards set by government. The biochemical test results received from all four laboratories revealed that on an average of four laboratories and two years, the trait purity range recorded was from 42 to 96 percent, whereas, quantification of Bt toxin ranged from 0.74 to 2.62. From the present study, it was concluded that almost 15-20 candidate varieties have the potential to be included among already approved varieties for commercial cultivation in the province of the Punjab.

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Abstract The associations between immune-related conditions and multiple myeloma (MM) and monoclonal gammopathy of undetermined significance (MGUS) have previously been investigated with inconsistent results. In a large population-based study, we identified 19 112 patients with MM, 5403 patients with MGUS, 96 617 matched control subjects, and 262 931 first-degree relatives. We calculated odds ratios (ORs) and 95% confidence intervals (CIs) for the association of MM and MGUS with immune-related conditions by use of logistic regression. A personal history of all infections combined was associated with a significantly increased risk of MM (OR = 1.2; 95% CI, 1.1-1.3), and a personal history of all conditions in the categories infections (OR = 1.6; 95% CI, 1.5-1.7), inflammatory conditions (OR = 1.4; 95% CI, 1.2-1.5), and autoimmune diseases (OR = 2.1; 95% CI, 1.9-2.4) was associated with a significantly increased risk of MGUS. Several specific immune-related conditions elevated the risk of MM and/or MGUS. A family history of autoimmune disease was associated with a significantly increased risk of MGUS (OR = 1.1; 95% CI, 1.00-1.2), but not MM. Our findings suggest that immune-related conditions and/or their treatment are of importance in the etiology of MGUS and possibly MM. The association of both personal and family history of autoimmune disease with MGUS indicates the potential for shared susceptibility for these conditions.

Abstract:

У статті наведено результати статистичного аналізу питомої активності 222Rn у зразках питної води, відібраних з артезіанських свердловин в різних регіонах України протягом 2003 – 2021 рр. Робота виконана в лабораторії радіаційного моніторингу Державної установи «Інститут громадського здоров’я ім. О.М. Марзєєва НАМН України». Періоди усереднення результатів вимірювань (три та чотири роки) пов’язані з вимогами нормативних актів та охопленням зразків води з географічно нових, не охоплених раніше, територій України. Наведено середні арифметичні значення та середньоквадратичні відхилення, а також середні геометричні, медіанні, мінімальні та максимальні значення питомої активності 222Rn у 1228 зразках питної води з артезіанських свердловин. Значення середньоквадратичних відхилень результатів вимірювань та близькість медіанних та середніх геометричних значень свідчать про логнормальний характер розподілу питомої активності 222Rn у вибірці. Встановлено, що для 11 % досліджених зразків води вміст 222Rn перевищує встановлений державний норматив для питної води (100 Бк/л). Виявлені високі рівні 222Rn мають відношення до окремих територій, де необхідне більш детальне вивчення радіоактивності підземних вод з метою підготовки та реалізації заходів для поліпшення радіаційного стану питної води. Порівняння результатів дослідження питомої активності 222Rn у питній воді за 2003 – 2021 рр. з відповідними даними за 1989 – 1991 рр. вказує на їх суттєву відмінність: для періоду 1989 – 1991 рр. середні арифметичні та середні геометричні значення значно вищі. Це пов’язано з тим, що відбірка у 1989 – 1991 рр. була більш випадковою, сконцентрованою переважно на території кристалічного щита та меншою мірою – поза його межами. Водночас дані 2003 – 2021 рр. охоплюють значно більшу кількість регіонів, а в розрахунки внесено повторні (з трирічним періодом) результати вимірювань 222Rn для деяких джерел. З метою встановлення чіткого стану радіоактивності питної води в Україні та з метою мінімізації опромінення людей від її споживання моніторинг вмісту 222Rn у питній воді для окремих регіонів України має бути поширений на джерела води, які залишаються недослідженими.

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ABSTRACT Background Endoscopic mucosal resection (EMR) is an easy-to-use treatment option for superficial colorectal lesions, including lesions ≥20 mm. Objective To evaluate the effectiveness of EMR. Methods We evaluated 430 lesions removed by EMR in 404 patients. The lesions were analyzed according to their morphology, size, location, and histology. Lesions <20 mm were resected en bloc, whereas lesions ≥20 mm were removed by piecemeal EMR (p-EMR). Adverse events and recurrence were assessed. Results Regarding morphology, 145 (33.7%) were depressed lesions, 157 (36.5%) were polypoid lesions and 128 (29.8%) were laterally spreading lesions, with 361 (84%) lesions <20 mm and 69 (16%) ≥20 mm. Regarding histology, 413 (96%) lesions were classified as neoplastic lesions. Overall, 14 (3.3%) adverse reactions occurred, most commonly in lesions removed by p-EMR (P<0.001) and associated with advanced histology (P=0.008). Recurrence occurred in 14 (5.2%) cases, more commonly in lesions removed by p-EMR (P<0.001). Conclusion EMR is an effective technique for the treatment of superficial colorectal lesions, even of large lesions.

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O objetivo do trabalho foi isolar micro-organismos de diferentes nichos e avaliar a capacidade dos mesmos no controle de patógenos invitro (Sclerotinia sclerotiorum e Fusarium solani) e in vivo (S. sclerotiorum), além da promoção do crescimento de Crotalaria sp. Asamostras de solo foram pesadas e diluídas para obtenção dos micro-organismos. Nos testes in vitro, as colônias bacterianas, que apresentaram crescimento, foram submetidas ao confrontamento direto com os fungos fitopatogênicos. Para análise fisiológica das sem*ntes de Crotalaria sp, as sem*ntes foram distribuídas em caixas gerbox contendo papel filtro umedecido com água destilada e incubadas a 20-30 ºC por 10 dias. Também foram testadas a antibiose de escleródios em meio líquido. O delineamento usado foi DIC e os dados foram submetidos à análise de variância e comparados por Tukey a 0,5%. Além disso, foi realizada a patologia de sem*ntes tratadas com as bactérias Fit-03 e Fit-04, além da microbiolização das sem*ntes para avaliação da promoção do crescimento em casa de vegetação. Dos 51 isolados, seis foram capazes de produzir substâncias capazes de inibir o crescimento micelial do F. solani e três de S. sclerotiorum. Na avaliação da capacidade de controle de micro-organismos sobre as sem*ntes das duas espécies de Crotalaria sp, Fit-03 e Fit-04 reduziram a incidência de fungos como Aspergillus e Penicillium. Pode-se afirmar que os isolados Fit-03 e Fit-04 apresentam potencial de uso no controle biológico do mofo branco em plantas de Crotalaria sp., bem como amplo espectro de ação.Palavras-chave: Antibiose. Sclerotinia sclerotiorum. Microbiolização de sem*ntes.AbstractThe objective was to isolate microorganisms of different niches and assess the ability of these in vitro control pathogens (Sclerotinia sclerotiorum and Fusarium solani) and in vivo (S. sclerotiorum) in addition to promoting the growth of Crotalaria sp. Soil samples were weighed and diluted to obtain the microorganisms. In in vitro tests the bacterial colonies that grew were subjected to direct confrontation with the pathogenic fungi. For physiological seed analysis Crotalaria sp. the seeds were distributed in gerbox boxes containing filter paper moistened with distilled water and incubated at 20-30 ° C for 10 days. Antibiosis sclerotia were also tested in liquid medium. The design used was DIC and the data were subjected to analysis of variance and compared by Tukey 0.5%. In addition, there was the seed pathology treated with the Fit-03 bacteria and Fit-04, in addition to microbiolization seeds for evaluation of growth promotion in greenhouse and also spraying the same in detached leaves of C. junceae and C. spectabillis and pathogen inoculation. After 51 isolates, six were able to produce substances capable of inhibiting the mycelial growth of F. solani and three S. sclerotiorum. In the evaluation of microorganisms control capability on the seeds of the two species of Crotalaria sp, Fit-Fit-03 and 04 reduced the incidence of fungi such as Aspergillus and Penicillium. It can be said that the Fit-03 isolated and Fit-04 have potential use in biological control of white mold in plant Crotalaria sp., as well as a broad spectrum of action.Keywords: Antibiosis. Sclerotinia sclerotiorum. Microbiolization seeds.

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Shared strains of Pseudomonas aeruginosa are now well recognized in people with cystic fibrosis (CF), and suitable P. aeruginosa laboratory typing tools are pivotal to understanding their clinical significance and guiding infection control policies in CF clinics. We therefore compared a single-nucleotide polymorphism (SNP)-based typing method using Sequenom iPLEX matrix-assisted laser desorption ionization with time-of-flight mass spectrometry (MALDI-TOF MS) with typing methods used routinely by our laboratory. We analysed 617 P. aeruginosa isolates that included 561 isolates from CF patients collected between 2001 and 2009 in two Brisbane CF clinics and typed previously by enterobacterial repetitive intergenic consensus (ERIC)-PCR, as well as 56 isolates from non-CF patients analysed previously by multilocus sequence typing (MLST). The isolates were tested using a P. aeruginosa Sequenom iPLEX MALDI-TOF (PA iPLEX) method comprising two multiplex reactions, a 13-plex and an 8-plex, to characterize 20 SNPs from the P. aeruginosa housekeeping genes acsA, aroE, guaA, mutL, nuoD, ppsA and trpE. These 20 SNPs were employed previously in a real-time format involving 20 separate assays in our laboratory. The SNP analysis revealed 121 different SNP profiles for the 561 CF isolates. Overall, there was at least 96 % agreement between the ERIC-PCR and SNP analyses for all predominant shared strains among patients attending our CF clinics: AUST-01, AUST-02 and AUST-06. For the less frequently encountered shared strain AUST-07, 6/25 (24 %) ERIC-PCR profiles were misidentified initially as AUST-02 or as unique, illustrating the difficulty of gel-based analyses. SNP results for the 56 non-CF isolates were consistent with previous MLST data. Thus, the PA iPLEX format provides an attractive high-throughput alternative to ERIC-PCR for large-scale investigations of shared P. aeruginosa strains.

Abstract:

An automated process that incorporates Millipore's Plasmid Miniprep96 Montáge™ Kit with the Apogent Discoveries PlateMate Plus® and Tango™ automated high-throughput dispensing systems has been developed for purifying plasmid DNA. To test the efficacy of this process, parameters such as the reproducibility and consistency of the purified DNA quantity and quality as well as the purification speed were analyzed. The purification time for two plates of the Plasmid Miniprep96 Kit (192 samples) was approximately 60 minutes using a PlateMate Plus equipped with 96 disposable tips and the Tango system equipped with 96 RB (resin bead) syringes. High uniformity and consistency in DNA yields (determined by spectrophotometric analysis) and quality (determined by gel electrophoresis analysis) among the different wells were observed. The purified plasmid DNA samples sequenced at an exceptional level with an average PHRED Q> 20 of 819 ± 25.

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RESUMO - O experimento foi realizado para avaliar a produção animal e o resultado econômico das misturas de aveia preta + azevém (MAA), triticale + azevém (MTA) e centeio + azevém (MCA) em pastejo contínuo. Foram usadas bezerras com sete meses de idade e 140 kg PV inicial. A semeadura foi realizada, a lanço, em 12 de abril de 1996, utilizando 85 kg/ha de aveia preta, 170 kg/ha de triticale, 140 kg/ha de centeio e 30 kg/ha de azevém, nas respectivas misturas. A área foi adubada com 200 kg/ha da fórmula 05-20-20 e a adubação nitrogenada foi de 220 kg/ha de nitrogênio. O pastejo ocorreu no período de 21/05/96 a 19/11/96, na MAA e MCA, e de 04/06/96 a 19/11/96, na MTA. A produção total de matéria seca (MS) foi semelhante entre as misturas, 9715, 9752 e 9770 kg de MS/ha, respectivamente. Houve interação entre tratamento e período de pastejo para ganho de peso médio diário e carga animal. O ganho de peso vivo/ha foi de 802,7 kg para a MTA, 753,9 kg para a MCA e 726,3 kg para a MAA. A receita líquida para a MTA foi de R$ 224,76, sendo 27,52% e 15,58% superior em relação à MAA e MCA, respectivamente.

Abstract:

RESUMO - O objetivo do experimento foi avaliar a dinâmica, produção e qualidade de forragem das misturas de aveia preta + azevém (MAA), triticale + azevém (MTA), centeio + azevém (MCA) em pastejo contínuo. Foram usadas bezerras com sete meses de idade inicial e 140 kg PV inicial. A semeadura foi realizada, a lanço, em 12 de abril de 1996, com 85 kg/ha de aveia preta, 170 kg/ha de triticale, 140 kg/ha de centeio e 30 kg/ha de azevém, nas respectivas misturas. A área foi adubada com 200 kg/ha da fórmula 05-20-20 e a adubação nitrogenada foi de 220 kg/ha de nitrogênio. O pastejo ocorreu no período de 21/05/96 a 19/11/96, na MAA e MCA, e de 04/06/96 a 19/11/96, na MTA. Houve interação entre tratamento e período de pastejo para as variáveis DIVMO e porcentagem de MS no resíduo. A taxa de acúmulo diário de MS e a produção total de MS não diferiram entre as misturas, apresentando produção de 9715, 9752 e 9770 kg de MS/ha, respectivamente. A mistura MCA apresentou maior produção de MS logo após o estabelecimento e a MAA, nos períodos iniciais de pastejo; já a MTA apresentou melhor distribuição da produção de forragem durante o período de pastejo. Houve maior contribuição inicial do centeio no resíduo, seguido pela aveia preta e pelo triticale.

Abstract:

The rubber seeds are insufficient for producing rootstocks to rubber grafting. It can be overcome by an in vitro micro-cutting culture technique developed in the Indonesian Research Institute for Biotechnology and Bioindustry (IRIBB). However, the origin clone of 57 rubber genotypes used as an explant source in vitro micro-cutting culture is not recognized. The study was to investigate the 57 genotypes that came from mixed GT 1, PB 260, and RRIM 600 as parent clones. We investigated using seven primers of Random Amplified Polymorphic DNA (RAPD), i.e., OPA 02, OPA 07, OPA 15, OPB 04, OPC 05, OPC 11, and OPC 20. The qualitative analyzed by electrophoresis 1% gel agarose. A total of 47 DNA fragments produced with an average of 7 fragments per primer. OPA 02 generated of 13 fragments, whereas OPB 04 only one fragment. The DNA fragment pattern shows the presence of polymorphism. The genetic similarity coefficients obtained in the range of 62-96%. The highest genetic similarity (96%) is genotype 70 and 78. It recognized that 42 genotypes from 57 rubber genotypes had the closest relationship with PB 260 clones. Furthermore, six genotypes had a significant growth response as an explant in vitro micro-cutting culture.

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The paper presents experimental study results of the cytopathic effect of peste de petit* ruminants virus on a goat gonad continuous cell line (YaDK-04). The interaction of peste de petit* ruminants virus with cells at different stages of its reproduction was shown using a combination of phase-contrast and luminescent microscopy. It was found that at the initial stage of interaction (20–24 hours) the cells became rounded and de-adhered, and the monolayer was partially loosened. On day 2 post reproduction the most part of the culture monolayer affected by the virus began to destruct, and the cell nuclei were displaced to periphery. At the terminal stage (72 hours) the destruction of monolayer cells and cytoplasmic matrix, deformation and partial lysis of the nuclei and cytoplasm, aggregation of detritus occurred. At the final stage of reproduction (96 hours) the peste de petit* ruminants virus diffused into the culture medium, the fluorescence in the yellow spectrum decreased significantly, but the virus titer reached 6.89 lg TCD50/cm3.

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We describe a reagent system and robotic methods for the purification of BigDye™ Terminator sequencing reactions prior to automated fluorescent sequence analysis. The methods use MagneSil™ paramagnetic particles to isolate sequencing extension products from unincorporated dye-labeled terminators and exchanges sequencer loading solution for reaction buffer. Processed samples give usable data that is greater than 98% accurate from primer plus 5–15 bases to over 700 bases. Typical Phred greater than 20 quality scores range from 600 to over 700 bases. This process has been adapted to a number of liquid handling robotic platforms in both 96- and 384-well formats. One method using a single POD Beckman Biomek® FX can process up to four plates in approximately 40 minutes.

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AbstractObjectiveSafety of home fortificants in children is uncertain in areas where infections are common. We tested the hypothesis that provision of lipid-based nutrient supplements (LNS) containing Fe does not increase infectious morbidity in children.DesignRandomized controlled trial. Infants were randomised to receive 10, 20 or 40 g LNS/d; or no supplement until age 18 months. All LNS contained 6 mg Fe/d. Morbidity outcomes (serious adverse events, non-scheduled visits and guardian-reported morbidity episodes) were compared between control and intervention groups using a non-inferiority margin of 20 %.SettingNamwera and Mangochi catchment areas in rural Malawi.SubjectsInfants aged 6 months (n1932).ResultsThe enrolled 1932 infants contributed 1306 child-years of follow-up. Baseline characteristics were similar across groups. Compared with the control group, the relative risk (95 % CI) of serious adverse events was 0·71 (0·48, 1·07), 0·67 (0·48, 0·95) and 0·91 (0·66, 1·25) in 10, 20 and 40 g LNS/d groups, respectively. The incidence rate ratio (95 % CI) of non-scheduled visits due to malaria was 1·10 (0·88, 1·37), 1·08 (0·89, 1·31) and 1·21 (1·00, 1·46), and of guardian-reported morbidity episodes was 1·04 (0·96, 1·11), 1·03 (0·97, 1·10) and 1·04 (0·97, 1·10), in the respective LNS groups.ConclusionsProvision of 10 and 20 g LNS/d containing 6 mg Fe/d did not increase morbidity in the children. Provision of 40 g LNS/d did not affect guardian-reported illness episodes but may have increased malaria-related non-scheduled visits.

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The aim of this study was to determine the most appropriate urine collection for detecting differences in the excretion rates of albumin, γ glutamyl transpeptidase (GGT) and N-acetyl-β-D-glucosaminidase (NAGA) between normotensive subjects and hypertensive patients on treatment. Twenty treated hypertensive patients, mean (SEM, standard error of mean) age; 52·2 (6·2) years and 20 normotensive subjects, mean age 49·2 (4·2) years, were studied in a consecutive sampling design. Urinary excretion rates of albumin, GGT and NAGA were determined in consecutive timed urine samples collected overnight and during 3–5 h the next morning. Mean (SEM) overnight excretion rates for albumin, GGT and NAGA for normotensive subjects were 11·05 (1· 18) μg/min, 17·00 (2·20)mU/min and 6·55 (0·39) mU/min, respectively, which were significantly lower than those of hypertensive subjects which were 20·77 (2·14) μg/min, 21·84 (1·65) mU/min and 10·92 (0·87) mU/min, respectively ( P < 0·05). The mean (SEM) percentage increases in urinary albumin, GGT and NAGA in morning urine collections of normotensive subjects of 15·22 (3·88)%, 34·04 (6·45)% and 11·54 (3·63)%, respectively were significantly lower than 107·03 (15·04)%, 121·96 (16·71)% and 72·75 (7·50)% found in hypertensive patients ( P < 0·05). These data suggest that were urinary albumin and tubular enzyme excretion to be used as correlates of hypertensive renal damage, ambulatory urine collections may be more sensitive than overnight collections.

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We describe a reagent system and robotic method for purifying plasmid DNA for restriction digestion, PCR, and fluorescent sequencing applications. The method uses two types of Wizard® MagneSil™ paramagnetic particles. Following lysis and neutralization procedures, the first particle type binds and removes cell debris; the second type is then used to bind plasmid DNA from the cleared lysate. The particles are then washed to eliminate unwanted contaminants. Purified plasmid DNA is then eluted from the particles with nuclease free water. When using a cell mass of approximately 4 O.D.600, the yield is 10–12μg of DNA when using high copy number plasmid. When used in BigDye® terminator sequencing, these DNA templates typically yield read lengths greater than 700 bases and Phred 20 scores of 600 to 750 bases. This purification method has been adapted for use on several robotic platforms in a 96-well format.

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Abstract BACKGROUND Factors associated with meningioma recurrence after postoperative radiotherapy are poorly understood, and the optimal postoperative radiotherapy target delineation for meningioma is unknown. The objective of this study was to identify factors influencing meningioma recurrence after postoperative radiotherapy to inform patient selection and treatment design. METHODS Medical records were retrospectively reviewed for patients who underwent meningioma resection at a single institution between 1991 and 2015. Patients with sufficient tumor tissue for histologic classification and who received postoperative radiation therapy with external beam radiotherapy (EBRT), stereotactic radiosurgery (SRS) or brachytherapy, were included. Local freedom from recurrence (LFFR) was analyzed according to tumor and treatment characteristics using the Kaplan Meier method. RESULTS We identified 86 patients with 96 meningiomas who met inclusion criteria. Nineteen meningiomas (20%) were WHO grade I, 56 (58%) were grade II and 21 (22%) were grade III. Forty-one meningiomas (43%) were recurrent, and 55 (57%) were de novo. The postoperative radiotherapy modality was EBRT for 58 patients (60%), SRS for 20 (21%) patients and brachytherapy for 18 (19%) patients. With a median follow up of 4.3 years (IQR 2.1–8.8 years), there were 48 (50%) local failures that occurred a median of 17 months after immediate prior resection (IQR 9–33 months). WHO grade II/III and recurrent meningiomas had worse LFFR (p< 0.001). The 5-year LFFR was 53% after EBRT (95% CI 41–69%), 53% after SRS (95% CI 34–84%) and 15% after brachytherapy (95% CI 3–74%), although meningiomas that were treated with brachytherapy were significantly more likely to have received prior EBRT or SRS (86% versus 29%, p< 0.001). CONCLUSIONS These data provide a foundation for understanding patterns of meningioma recurrence after postoperative radiotherapy. Ongoing analyses aim to quantify the relationships between postoperative radiotherapy dose, target delineation and local control of meningioma.

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Introduction: Patients with newly diagnosed AML are increasingly being enrolled on industry-sponsored clinical trials. These trials routinely exclude patients with Zubrod performance status (PS) >2, bilirubin >1.9 mg/dL, or creatinine >1.9 mg/dL; such patients are well-known to be relatively unlikely to respond (UTR). Here we quantify the effect of this practice on inclusion of UTR patients in clinical trials. Patients and Methods: Overall, 2323 adults with newly-diagnosed AML (no acute promyelocytic leukemia) were registered on clinical trials (industry-sponsored and other) from 1991–2006. Considering patients as UTR if they fulfilled any of the criteria noted above, we examined rates of enrollment of UTR patients over time: Results: Thus, while more patients are being enrolled on clinical trials, fewer UTR patients are being enrolled particularly over the last 5 years (p = .04) with a concomitant decrease in the proportion of such patients enrolled (p<.0001 over the 16 years in question). Findings are qualitatively the same in older patients. Conclusion: Newer clinical trials may be less applicable to UTR patients than previous trials. There is a need for clinical trials specific to the UTR population, the group most in need of novel therapies. Rates of enrollement of unlikely to respond patients on clinical trials for newly diangosed AML Year No. of pts. treated on clinical trial (pts. age≥60 yrs) UTR pts. enrolled (pts. age≥60 yrs) % of UTR pts. enrolled (pts. age ≥60 yrs) 1991 101 (50) 17 (13) 17 (26) 1992 100 (48) 20 (14) 20 (29) 1993 96 (46) 18 (9) 19 (20) 1994 109 (41) 23 (13) 21 (32) 1995 102 (48) 20 (10) 20 (21) 1996 104 (63) 14 (10) 13 (16) 1997 145 (81) 30 (20) 21 (25) 1998 124 (63) 14 (8) 11 (13) 1999 164 (86) 26 (16) 16 (19) 2000 169 (96) 25 (19) 15 (20) 2001 187 (98) 30 (19) 16 (19) 2002 162 (91) 17 (11) 10 (12) 2003 195 (111) 4 (2) 2 (2) 2004 184 (109) 17 (11) 9 (10) 2005 185 (117) 15 (7) 8 (6) 2006 196 (109) 17 (12) 9 (11) Total 2323 (1257) 307 (194) 13 (15)

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BackgroundEvidence from observational studies suggests an increased risk of HIV acquisition among women using depot medroxyprogesterone acetate (DMPA) contraception.MethodsWithin the context of a South African programme to increase women's access to the intrauterine contraceptive device (IUD), we conducted a pragmatic, open-label, parallel-arm, randomised controlled trial (RCT) of the IUD versus injectable progestogen contraception (IPC) at two South African hospitals. The primary outcome was pregnancy; secondary outcomes included HIV acquisition. Consenting women attending termination of pregnancy services were randomised after pregnancy termination between July 2009 and November 2012. Condoms were promoted for the prevention of sexually transmitted infections. Voluntary HIV testing was offered at baseline and at 12 or more months later. Findings on HIV acquisition are reported in this article.ResultsHIV acquisition data were available for 1290 initially HIV-negative women who underwent a final study interview at a median of 20 months after randomisation to IPC or an IUD. Baseline group characteristics were comparable. In the IPC group, 545/656 (83%) of participants received DMPA, 96 (15%) received injectable norethisterone enanthate, 14 (2%) received the IUD and one received oral contraception. In the IUD group 609 (96%) received the IUD, 20 (3%) received IPC and 5 (1%) had missing data. According to intention-to-treat analysis, HIV acquisition occurred in 20/656 (3.0%) women in the IPC arm and 22/634 (3.5%) women in the IUD arm (IPC vs IUD, risk ratio 0.88; 95% confidence interval 0.48–1.59;p=0.7).ConclusionsThis sub-study was underpowered to rule out moderate differences in HIV risk, but confirms the feasibility of randomised trial methodology to address this question. Larger RCTs are needed to determine the relative risks of various contraceptive methods on HIV acquisition with greater precision.Trial registration numberPan African Clinical Trials Registry number PACTR201409000880157 (04-09-2014).

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Abstract Purpose To report our experience with the delivery of passively scattered proton therapy in the management of nonmelanoma skin cancers with clinical perineural invasion. Materials and Methods We reviewed the medical records of patients who received definitive or postoperative proton therapy for nonmelanoma skin cancer with clinical perineural invasion at our institution and updated patient follow-up when possible. All patients were treated with curative intent with or without the delivery of concurrent systemic therapy. We report disease control rates and the rates of late toxicity among this cohort. Results Twenty-six patients treated between 2008 and 2017 were included in the analysis. Following proton therapy, the 3-year overall, cause-specific, and disease-free survival rates were 59%, 73%, and 60%, respectively. The 3-year local control, local regional control, and distant metastasis-free survival rates were 80%, 65%, and 96%, respectively. On univariate analysis, surgical resection before radiation therapy significantly improved local regional control rates at 3 years (55% versus 86%; P = .04). Grade 3+ late toxicities occurred in 13 patients (50%) and the most common toxicities included grade 3+ keratitis of the ipsilateral eye, which occurred in 4 patients (15%) and grade 3+ brain necrosis in 4 patients (15%). Conclusion Proton therapy is effective in the management of nonmelanoma skin cancer with clinical perineural invasion. Although disease control and complication rates compare favorably to those previously published for photon-based radiation therapy, the risk for late toxicity is significant and patients should be appropriately counseled.

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Abstract BACKGROUND CNS germ cell tumors represent about 3–5 % of pediatric brain tumors, 60% are pure germinomas. Germinomas are very sensitive to chemotherapy which has helped to reduce volume and dose of radiotherapy in localized disease while maintaining excellent survival. In metastatic disease the SIOP GCT-96 trial showed no benefit with addition of chemotherapy to craniospinal irradiation alone. Radiotherapy maybe not readily available in Low/Middle Income Countries (LMIC). METHOD We describe a patient in which the use of neoadjuvant chemotherapy helped to rescue vision. The patient is a 9 year old female with a 3 months history of morning headaches and vomits. Visual decline was noticed a month before admission when the child had completely loss vision of the right eye and left eye was partially affected. MRI showed a large suprasellar mass with ventricular nodules. Beta-hGC in CSF was mildly elevated. Patient received 2 cycles of carboplatin/etoposide. After first cycle there was a complete vision recovery in both eyes. After the second course the MRI showed complete response in primary and metastatic disease. Patient received CSI (24Gy + 16 Gy Boost) after 2 cycles of chemotherapy. Chemotherapy was very well tolerated without side effects. Patient vision is 20/20 in both eyes without deficit in visual fields. CONCLUSION Although the addition of chemotherapy in metastatic germinoma has no clear role in reducing radiotherapy it could possible help selected patients in attempt to rescue vision when radiotherapy is not readily available.

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AbstractMaintenance haemodialysis (MHD) is the use of a machine to filter wastes, salts and fluid from blood for at least 3 months to prolong the life of patients with advanced kidney failure. Although low dietary energy intake (DEI) has been observed in MHD patients, few studies have related DEI to the risk of mortality. To explore this relationship, a study included 1039 MHD patients from eight centres was conducted. DEI was assessed by three 24-h diet recalls and was normalised to ideal body weight (IBW). All-cause mortality and CVD mortality were the primary and secondary outcomes, respectively. During a median follow-up of 28 months, a U-shaped relationship was observed between DEI and all-cause or CVD mortality. The risk of all-cause mortality decreased significantly with the increase of DEI in participants with DEI <167·4 kJ/kg IBW per d (hazard ratio (HR) 0·98; 95 % CI 0·96, 1·00) and increased significantly with the increase of DEI in those with DEI ≥167·4 kJ/kg IBW per d (HR 1·12; 95 % CI 1·04, 1·20). Similarly, the risk of CVD mortality decreased with the increase of DEI in participants with DEI <152·7 kJ/kg IBW per d (HR 0·96; 95 % CI 0·93, 0·99) and increased with the increase of DEI in participants with DEI ≥152·7 kJ/kg IBW per d (HR 1·11; 95 % CI 1·04, 1·18). In summary, there was a U-shaped association between DEI and all-cause or CVD mortality, with a turning point at about 167·4 and 152·7 kJ/kg IBW per d, respectively, in MHD patients.

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Abstract Oncogenic pathways underlying in the development of myelodysplastic syndromes (MDS) remain poorly characterized, but mutations of the ten-eleven translocation 2 (TET2) gene are frequently observed. In the present work, we evaluated the prognostic impact of TET2 mutations in MDS. Frameshift, nonsense, missense mutations, or defects in gene structure were identified in 22 (22.9%) of 96 patients (95% confidence interval [CI], 14.5-31.3 patients). Mutated and unmutated patients did not significantly differ in initial clinical or hematologic parameters. The 5-year OS was 76.9% (95% CI, 49.2%-91.3%) in mutated versus 18.3% (95% CI, 4.2%-41.1%) in unmutated patients (P = .005). The 3-year leukemia-free survival was 89.3% (95% CI, 63.1%-97.0%) in mutated versus 63.7% (95% CI, 48.2%-75.4%) in unmutated patients (P = .035). In univariate analysis (Cox proportional hazard model), the absence of TET2 mutation was associated with a 4.1-fold (95% CI, 1.4-12.0-fold) increased risk of death (P = .009). In multivariate analysis adjusted for age, International Prognostic Scoring System, and transfusion requirement, the presence of TET2 mutation remained an independent factor of favorable prognosis (hazard ratio, 5.2; 95% CI, 1.6-16.3; P = .005). These results indicate that TET2 mutations observed in approximately 20% of patients, irrespective of the World Health Organization or French-American-British subtype, represent a molecular marker for good prognosis in MDS.

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Abstract Mediastinal large B-cell lymphoma (MLBL) represents 2% of mature B-cell non-Hodgkin lymphoma in patients ≤ 18 years of age. We analyzed data from childhood and adolescent patients with stage III MLBL (n = 42) and non-MLBL DLBCL (n = 69) treated with Group B therapy in the French-American-British/Lymphome Malins de Burkitt (FAB/LMB) 96 study. MLBL patients had a male/female 26/16; median age, 15.7 years (range, 12.5-19.7); and LDH &lt; 2 versus ≥ 2 × the upper limit of normal, 23:19. Six MLBL patients (14%) had &lt; a 20% response to initial COP (cyclophosphamide, vincristine, and prednisone) therapy. Central pathology revealed approximately 50% with classical features of primary MLBL. Five-year event-free survival for the stage III MLBL and non-MLBL DLBCL groups was 66% (95% confidence interval [CI], 49%-78%) and 85% (95% CI, 71%-92%), respectively (P &lt; .001; 14%). The 5-year overall survival in the 42 MLBL patients was 73% (95% CI, 56%-84%). We conclude that MLBL in adolescent patients is associated with significantly inferior event-free survival compared with stage III non-MLBL DLBCL and can be of multiple histologies. Alternate treatment strategies should be investigated in the future taking into account both adult MLBL approaches and more recent biologic findings in adult MLBL.

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AbstractIron deficiency (ID) is the most prevalent micronutrient deficiency in the world, particularly in developing countries. Blood samples and a qualitative FFQ on Fe- and vitamin C-rich foods were obtained in 180 adolescent girls aged 12 to 17 years living in two boarding schools from south Benin. ID, defined as serum ferritin either <20μg/l or 20–50μg/l, plus two of the following parameters: serum Fe<11μmol/l, total iron-binding capacity>73μmol/l or transferrin saturation<20%, was found in 32% of subjects. Anaemia (Hb<120g/l) was found in 51% of adolescents, while 24% suffered from iron-deficiency anaemia (IDA) (ID and Hb<20g/l). After adjusting for confounding factors (age, mother's and father's occupation, household size) in a logistic regression equation, subjects having a low meat consumption (beef, mutton, pork) (<4 times/week) were more than twice as likely to suffer from ID (OR=2·43; 95% CI 1·72, 3·35;P=0·04). Adolescents consuming less fruits (<4 times/week) also had a higher likelihood of suffering from ID (OR=1·53; 95% CI 1·31, 2·80;P=0·03). Finally, subjects whose meat consumption was low were twice as likely to suffer from IDA (OR=2·24; 95% CI 1·01, 4·96;P=0·04). The prevalence of ID represents an important health problem in these Beninese adolescent girls. A higher consumption of Fe-rich foods and of promoters of Fe absorption (meat factor and vitamin C) is recommended to prevent ID deficiency in these subjects.

Abstract:

Globodera pallida is a major nematode pest of potato (Solanum tuberosum) and is of great economic importance for the potato industry. Assessing potato yield loss caused by the Idaho G. pallida population under field conditions was not performed due to its quarantine status in Idaho, where it is prohibited by regulatory statutes to grow potato in any infested fields. The experimental data came from three trials that were conducted under greenhouse conditions. A predictive risk model analysis was performed to: (i) determine the effect of the Idaho population of G. pallida on potato yield; (ii) estimate reproduction rate from different initial nematode densities; and (iii) simulate potato yield losses in Idaho field conditions by integrating the coefficients of potato yield into the SUBSTOR-DSSAT crop simulation model. Experiments were conducted under greenhouse conditions using five initial G. pallida soil infestation levels (0, 10, 20, 40, and 80 eggs/g soil). The coefficients of potato yield achieved under each initial nematode density were integrated into the SUBSTOR-DSSAT potato growth simulation model. The model showed that tuber weight reached a maximum yield of 96 ton/ha in noninfested soil. Based on the greenhouse trials, the model predicted a minimum yield of 12 and 58 ton/ha in trial 1 and trial 2/3 respectively, when initial nematode density was 80 eggs/g soil. In trial 1, tuber weight was significantly reduced by 44% at 40 eggs/g soil and by 87% at 80 eggs/g soil, and 20% at 40 eggs/g soil and by 39% at 80 eggs/g soil in trial 2/3. The outputs of this study should facilitate common understanding between regulators, policymakers, and potato growers on the challenges and opportunities for controlling this economically important pest in Idaho.

Abstract:

Growth chamber and field studies were conducted with organic amendment mixtures of carbon (C) and nitrogen (N) at C:N ratios 10:1, 20:1, 30:1, and 40:1 and amendment rates of C at 2, 4, 6, and 8 mg/g of soil (C:N ratio 30:1) to evaluate anaerobic soil disinfestation (ASD) effects on germination and colonization of Sclerotium rolfsii. In the growth chamber, sclerotial germination was reduced in all ASD treatments regardless of C:N ratio (0.6 to 8.5% germination) or amendment rate (7.5 to 46%) as compared with nonamended controls (21 to 36% and 61 to 96%, respectively). ASD treatment increased Trichoderma spp. colonization of sclerotia, with consistently higher colonization in ASD treatments with amendment rates of C at 2 or 4 mg/g of soil (>87% colonization) compared with nonamended controls (<50% colonization). In the 2014 field study, sclerotial germination was reduced by 24 to 30% in ASD treatments when compared with the nonamended control. Sclerotial colonization by Trichoderma spp. was predominant; however, other potential mycoparasites (i.e., Aspergillus spp., Fusarium spp., zygomycetes, and other fungi) were present in the field study. Amendment C:N ratios in the range of 10:1 to 40:1 were equally effective in reducing sclerotial germination and enhancing colonization by potentially beneficial mycoparasites of sclerotia.

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El objetivo fue evaluar los parámetros de proceso y su influencia en las características físico-químicas y organolépticas de un pisco mosto verde. Del proceso fermentativo incompleto se obtuvieron cuatro (04) muestras transcurridas 84, 96, 108 y 120 horas, a las que se evaluó en acidez total, densidad, temperatura, pH, sólidos solubles, (°Brix) y grado alcohólico (% vol). En la destilación se separaron tres fracciones: "cabeza", un 0,9% del mosto a desfilar; "cuerpo" hasta alcanzar 43,0 +/- 0,5 % vol a 20°C en la mezcla hidroalcohólica; y "cola", el resto de la destilación. A la fracción "cuerpo" se le evaluó grado alcohólico, extracto seco, densidad y pH. Por cromatografía de gases se evaluarán los principales componentes químicos (ésteres, furfural, aldehídos, alcoholes superiores, acidez volátil, alcohol metílico, compuestos volátiles y odoríferos). El análisis estadístico, y de preferencia hedónica para la evaluación organoléptica, dio como resultado no existir diferencia significativa entre las cuatro muestras a un nivel de 95 % de confianza. Se confirmó que todas las muestras evaluadas cumplían con los requisitos de la Norma Técnica Peruana N°211.001 (2002). Pisco. Requisitos.

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Magnetic resonance imaging (MRI) signs of elevated intracranial pressure and idiopathic intracranial hypertension have been well characterized in adults but not in children. The MRIs of 50 children with idiopathic intracranial hypertension and 46 adults with idiopathic intracranial hypertension were reviewed for optic nerve head protrusion, optic nerve head enhancement, posterior scleral flattening, increased perioptic cerebrospinal fluid, optic nerve tortuosity, empty or partially empty sella, tonsillar herniation, enlargement of Meckel’s cave meningoceles, and transverse venous sinus stenosis(TSS). Compared to adolescents (11-17 years, n = 40) and adults (>17 years, n = 46), prepubescent children (<11 years, n = 10) had lower frequencies of scleral flattening (50% vs 89% and 85%, P = .02), increased perioptic cerebrospinal fluid (60% vs 84% and 89%, P = .08), optic nerve tortuosity (20% vs 46% and 59%, P = .07), empty or partially empty sella (56% vs 78% and 93%, P = .007), and TSS (67% vs 93% and 96%, P = .04). Children with idiopathic intracranial hypertension have similar MRI findings as adults, but they are less frequent in prepubescent children.

Abstract:

The use of tomato (Solanum lycopersicum) grafting is gaining traction across the United States, but small-scale growers face the challenge of creating optimum postgrafting healing conditions. The practice of blocking light for a period of 2 to 4 days while maintaining high humidity is commonly recommended for healing grafted tomato transplants; however, research is exploring alternatives to this practice. The present study investigated a low-input healing method for grafted tomato transplants with a specific focus on light and the use of propagation heat mats to regulate substrate and healing chamber air temperatures during the 7-day healing process. We hypothesized that 4 days of light exclusion and the use of propagation heat mats would improve grafted tomato transplant survival and growth. ‘Cherokee Purple’ was used as the scion and ‘RST-04-106-T’ was used as the rootstock. The whole plot factor was heat [propagation mats set at 80 °F (heat) or no propagation mat (no heat)] and the subplot factor was light exclusion (0, 4, or 6 days of dark). The highest survival rate among treatments was 97% in 0 days of dark with no heat treatment; survival decreased to 84% in 4 and 6 days of dark with no heat treatments. The plant survival rate was 96% with 0 days of dark and heat treatment; however, the survival rates were 63% and 45% for the 4- and 6-day dark treatments, respectively. The scion stem diameter was largest for transplants grown in 0 days of dark, but there was no difference in stem diameter due to heat treatments. There were no differences among scion or rootstock biomasses due to heat or light treatments. These results demonstrate that propagation mats set at 80 °F to regulate the substrate temperature were detrimental to grafted transplant survival under extended periods of light exclusion. However, this finding creates the basis to explore lower levels of substrate temperature modification. Our work also indicates that light exclusion may not be necessary for healing grafted tomato plants regardless of root-zone temperature treatments. Future work should examine the interactions of various substrate and air temperatures under full light conditions and their effects on grafted tomato transplant survival and growth. This work contributes to the ongoing research of how to optimize low-input healing methods that may be readily adopted by small-scale tomato growers.

Abstract:

Abstract Radiotherapy (RT) can be curative in patients with localized follicular lymphoma (FL), with historical series showing a 10-year disease-free survival of 40 to 50%. As 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography with computerized tomography (PET-CT) upstages 10 to 60% of patients compared to CT, we sought to evaluate outcomes in patients staged by PET-CT, to determine if more accurate staging leads to better patient selection and results. We conducted a multicenter retrospective study under the direction of the International Lymphoma Radiation Oncology Group (ILROG). Inclusion criteria were: RT alone for untreated stage I to II FL (grade 1-3A) with dose equivalent ≥24 Gy, staged by PET-CT, age ≥18 years, and follow-up ≥3 months. End points were freedom from progression (FFP), local control, and overall survival (OS). A total of 512 patients treated between 2000 and 2017 at 16 centers were eligible for analysis; median age was 58 years (range, 20-90); 410 patients (80.1%) had stage I disease; median RT dose was 30 Gy (24-52); and median follow-up was 52 months (3.2-174.6). Five-year FFP and OS were 68.9% and 96%. For stage I, FFP was 74.1% vs 49.1% for stage II (P &lt; .0001). Eight patients relapsed in-field (1.6%). Four had marginal recurrences (0.8%) resulting in local control rate of 97.6%. On multivariable analysis, stage II (hazard ratio [HR], 2.11; 95% confidence interval [CI], 1.44-3.10) and BCL2 expression (HR, 1.62; 95% CI, 1.07-2.47) were significantly associated with less favorable FFP. Outcome after RT in PET-CT staged patients appears to be better than in earlier series, particularly in stage I disease, suggesting that the curative potential of RT for truly localized FL has been underestimated.

Abstract:

4549 Background: We began studies with HDCE for patients (pts.) with recurrent GCTs 20 years ago. During the past decade, better supportive care and use of PBSCT allowed outpatient therapy and more rapid hematopoietic recovery between the 2 courses of HDCE. Methods: Retrospective review of 184 consecutive pts. treated with HDCE at Indiana University from 2–96 to 12–04. Late relapse (> 2 years from prior therapy) and primary mediastinal non-seminomatous germ cell tumor pts. were not offered HDCE. Cytoreduction with 0–2 courses of vinblastine + ifosfamide + cisplatin preceded HDCE. C dosage was 700 mg/M2 × 3 and E 750 mg/M2 × 3. A second course was given after hematologic recovery. Results: Toxicity was as previously described (JCO 18:3346, 2000). There were 3 drug- related mortalities. An additional 3 patients developed AML (2 fatal), and 1 glioma following CNS XRT for metastases. 11 pts. did not receive second course (8 due to progression or HDCE mortality). Median time to second course HDCE was 28 days (range 20 to 42). 116 of 184 pts. are alive and continuously (cont) NED (63%) with median followup 42 months (range 11 to 118). 113 (97%) of these are 12+ months NED. 5 additional pts. are currently NED with further therapy. Results are tabulated below. Conclusions: HDCE has a high cure rate with acceptable toxicity as salvage therapy for GCT pts. [Table: see text] [Table: see text]

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AbstractObjectivePrior studies on linoleic acid, the predominant n-6 fatty acid, and breast cancer risk have generated inconsistent results. We performed a meta-analysis to summarize the evidence regarding the relationship of dietary and serum linoleic acid with breast cancer risk.DesignPertinent studies were identified by a search of PubMed and EMBASE. The fixed- or random-effect pooled measure was selected based on between-study heterogeneity.ResultsEight prospective cohort studies and four prospective nested case–control studies, involving 10 410 breast cancer events from 358 955 adult females across different countries, were included in present study. Compared with the lowest level of linoleic acid, the pooled relative risk (RR; 95 % CI) of breast cancer was 0·98 (0·93, 1·04) for the highest level of linoleic acid. The pooled RR (95 % CI) for dietary and serum linoleic acid were 0·99 (0·92, 1·06) and 0·98 (0·88, 1·08), respectively. The RR (95 % CI) of breast cancer was 0·97 (0·91, 1·04), 0·95 (0·85, 1·07), 0·96 (0·86, 1·07), 0·98 (0·87, 1·10) and 0·99 (0·85, 1·14) for linoleic acid intake of 5, 10, 15, 20 and 25 g/d, respectively. The risk of breast cancer decreased by 1 % (RR=0·99; 95 % CI 0·93, 1·05) for every 10 g/d increment in linoleic acid intake.ConclusionsThis meta-analysis indicated that both dietary linoleic acid intake and serum linoleic acid level were associated with decreased risk of breast cancer, although none of the associations were statistically significant. Further investigations are warranted.

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AbstractObjectiveTo determine if associations exist between a range of unsaturated fatty acid intakes and mental health outcomes.DesignCross-sectional data analysis of the Australian Longitudinal Study on Women’s Health (ALSWH) Young Cohort Survey 3 that included the validated seventy-four-item Dietary Questionnaire for Epidemiological Studies FFQ, validated mental health scales and self-report questions on depression and anxiety.SettingAustralia, 2003.SubjectsA nationally representative sample of young Australian women (25–30 years) from ALSWH. The 7635 women with plausible energy intakes (>4·5 but <20·0 MJ/d) were included in the analyses.ResultsAdjusted logistic regression analyses found statistically significant associations between higher intakes of α-linolenic acid and decreased likelihood of depressive symptoms indicated by the ten-item Center for Epidemiological Studies Depression Scale (CESD-10; OR=0·77; 95 % CI 0·60, 0·99; P=0·040) and the Short Form Health Survey (SF-36) mental health subscale (OR=0·73 95 % CI 0·56, 0·96; P=0·024). Furthermore, higher intakes of n-6 fatty acids (OR=0·96, 95 % CI 0·93, 0·99; P=0·019) and linoleic acid (OR=0·96, 95 % CI 0·93, 0·99; P=0·020) were associated with decreased likelihood of self-reported diagnosed anxiety and higher intakes of n-9 fatty acids (OR=1·02, 95 % CI 1·00, 1·04; P=0·041) and oleic acid (OR=1·02, 95 % CI 1·00, 1·05; P=0·046) were associated with increased likelihood of self-reported diagnosed anxiety.Conclusions:Increased intakes of α-linolenic acid were associated with a reduced likelihood of depressive symptoms, increased intakes of n-6 fatty acids and linoleic acid were associated with a reduced likelihood of self-reported anxiety, and increased intakes of n-9 fatty acids and oleic acid were associated with an increased likelihood of anxiety. Additional studies are needed to further elucidate associations between unsaturated fatty acids and depression and anxiety.

Abstract:

O trigo (Triticum aestivum L.) é uma das principais culturas no Brasil e a quantidade de nitrogênio a ela aplicada é um dos fatores que mais estimula sua produção, o que resulta num maior número de perfilhos, portanto maior número de plantas/m2 e, conseqüentemente, maior produtividade em kg/ha. O presente trabalho teve como principal objetivo avaliar os efeitos de adubação de nitrogênio em cobertura em relação ao número de espigas/m2, número de espiguetas por espigas, peso hectolitro, peso de 1000 grãos e produtividade da cultivar IPR118. O experimento foi conduzido na fazenda WS, localizada no município de Colombo – PR, no período de 10/04/2006 a 20/10/2006. O delineamento utilizado foi o de blocos ao acaso com 8 tratamentos e 4 repetições, sendo utilizado uréia como adubo de cobertura nas seguintes dosagens 0, 20, 40, 80, 160, 250, 350 e 450 kg/ha, T1, T2, T3, T4, T5, T6, T7 e T8, respectivamente. O maior rendimento foi obtido com o tratamento T6 (1445 kg/ha), entretanto esse não diferiu estatisticamente apenas do tratamento T8 (1436 kg/ha). O maior número de espigas/m2 foi obtido no tratamento T6 (96), não diferindo estatisticamente apenas do tratamento T7 (94,5). A análise de regressão mostrou um decréscimo no rendimento e número de espigas/m2 a partir da dose de 250 kg de N/ha. Não houve diferenças significativas quanto ao peso de 1000 grãos e peso hectolitro, porém no período do experimento ocorreu uma grande estiagem na época de germinação e geadas durante o florescimento.

Abstract:

8546 Background: Romidepsin is a novel pan-HDAC inhibitor with demonstrated single-agent activity in 2 open-label clinical studies of 167 patients (pts) with CTCL [mycosis fungoides or Sézary Syndrome (SS)]. Data for these 2 studies were pooled for more accurate estimates of endpoints and investigation of subpopulations. Methods: GPI-04–0001 (pivotal study) enrolled 96 pts with confirmed CTCL who had recieved ≥1 prior systemic therapy. NCI 1312 (supportive study) enrolled 71 pts with CTCL. Pts recieved romidepsin, 14mg/m2 as a 4-hr infusion on days 1, 8, and 15 every 28 days until disease progression (≥25% increase). The primary efficacy endpoint for both studies was overall response rate (ORR) using a composite endpoint that included skin assessment, lymph node and visceral involvement and abnormal circulating T-cells/Sézary cells. Results: 167 pts received romidepsin (as-treated); 135 pts (81%) were in the efficacy evaluable population. Mean age was 57±12 yrs, 67% men, 87% white. 103 pts (76%) had stage ≥IIB disease. Median number of prior systemic therapies was 2 (range 1–8). ORR in the table. Responses were noted in: 42% of pts with stage ≥IIB; 11 (58%) of 19 pts with SS (erythroderma + Sézary cells, >1000/ml or >20% ); and 20 (38%) of 52 pts who received prior bexarotene and 8 (40%) of 20 pts who had received denileukin diftitox. Most common drug-related adverse events (AE), all grades, included: nausea (67%), fatigue (49%), anorexia (37%), ECG T-wave changes (29%), anemia (26%), dysgeusia (23%), neutropenia (22%), leucopenia (20%). Related serious AEs in 2% of pts: supraventicular arrhythmia, ventricular arrhythmia, infection, neutropenia, WBC decreased, hyperuricemia, hypotension; all other serious AEs in ≤1 pt. 3 deaths reported as possibly related. Conclusions: Romidepsin is a valuable new therapy for pts with CTCL based on the ORR, CR, durability of response, improvement in all disease compartments and responses at all stages and in all subpopulations analyzed. [Table: see text] [Table: see text]

Abstract:

Aim: To compare the radiological and functional result of conservatively treated distant radius fractures (manipulation under anesthesia and plaster of paris) versus percutaneous K- wire fixation Study design: Retrospective Duration and place of study: Department of Orthopedic Surgery, The Indus Hospital, Raiwind campus Lahore from July 2018 to August 2020. Methods: The sample size of 100 patients aged between 18-60 years, closed, isolated distal radius fracture were included, and patients with diabetes mellitus, ischemic heart disease, pathological fractures, chronic renal disease, head injury, chronic liver disease, whose Glasgow coma scale was <14, malignancy were excluded. Patients were randomized as A & B by lottery method. They managed with conservative treatment ( manipulation under anesthesia (MUA) and plaster of paris (POP) cast) and K- wire fixation in groups A & B, respectively. Side of the fractured bone, infection, radiological union, and function outcome were recorded. Results: Amongst the total 100 patients in group-A, 32(63.8%) were males, and 18(36.2%) were females, and 35 (70.2%) were males, and 15 (29.8%) were females in Group-B. in group A patients mean age was 38.86.19 years, while patients mean age of the group B was 39.57.36 years. With the conservative method, 45(95.7%), the union rate was while with the K- wire fixation method, the union rate was 48(96%). In group-A, 35(70%) had excellent, 10 (20%) good, 03 (6%) had an average, and 2(4%) poor and in group-B, 40(80%) had excellent, 04 (08%) good, 04(08%) had an average, and 02 (4%) poor functional outcome. Conclusion: In terms of clinical and radiological evaluation, fixation of a distal radius fracture with K-wires has a better outcome than conservative treatment (MUA and POP cast) with a low infection rate and better union and result. Keywords: closed fracture, distal radius, back slab, K-wires

Abstract:

5507 Background: Despite resection and postoperative irradiation high-risk (3 or more involved lymph nodes, extra-capsular disease and/or microscopically involved mucosal margins of resection) squamous cell carcinomas (SCCAs) of the head and neck frequently recur in the tumor bed. Postoperatively radiochemotherapy (RCT) with cis-Platin (CDDP)/5-FU versus radiotherapy (RT) alone was compared in a randomized trial. Methods: Between 5/97 and 12/04, 440 patients who had high-risk SCCAs of the head and neck were enrolled in this prospectively randomized phase III trial. Following resection and neck dissection, 214 patients were randomly assigned to RT (66 Gy/33 Fx/6.6 weeks) and 226 patients to identical RT plus CDDP (20 mg/m2 on day 1–5, 29–33) and 5-FU (600 mg/m2 on day 1–5, 29–33). Results: The 5 year local-regional control rate is 72.2 ± 3.7% following RT and 88.6 ± 2.4% for the RCT group (p = 0.00259; 5-year progression free survival 50.1 ± 4.0% and 62.4 ± 4.4% (p = 0.024) and 5-year overall survival 48.6 ± 4.4% vs. 58.1 ± 4.6% (p = 0.11). There was no difference in the 5 year incidence of distant metastases (19.3 ± 3.6% vs 25.5 ± 4.6%; p = 0.45). The incidence of grade 3+ acute toxicity was higher during RCT: mucositis 12.6% vs. 20.8% (p = 0.04), leucopenia 0% vs. 4.4% (p = 0.007). Conclusions: Acute toxicity is increased to an acceptable level by RCT. Postoperative RCT compared to RT improves locoregional control and progression free survival; thus survival as a trend is improved by 10% after 5 years. Supported by Deutsche Krebshilfe 70–2140. No significant financial relationships to disclose.

Abstract:

Meloidogyne marylandi is a nematode commonly associated with turfgrasses and has been reported to occur in Texas and Arkansas (1,3). In the fall of 2013, a stand of ultradwarf bermudagrass (Cynodon dactylon × C. transvaalensis) plants in a sand-based, research putting green in Stillwater, Oklahoma, exhibited symptoms of decline. Roots of the affected plants had small galls and upon staining of the root system, numerous egg masses were evident. Egg masses were collected, placed in water, and the morphology of 20 hatched, second-stage juveniles were examined. The characteristics of the juveniles were: body length averaged 393.1 ± 19.87 (range: 361 to 425) μm, mean width averaged 16.6 ± 0.7 (15.6 to 17.8) μm, stylet lengths averaged 12.1 ± 0.7 (10.4 to 12.9) μm, dorsal gland orifice from stylet base averaged 2.9 ± 0.4 (2.5 to 3.6) μm, tail lengths averaged 53.7 ± 3.8 (46.2 to 60.4) μm, and the hyaline region of the tails averaged 10.4 ± 1.1 (8.4 to 12.7) μm. Genomic DNA was extracted from six females that were removed from roots. Amplification and sequencing of the mitochondrial DNA region between COII and 16S rRNA genes was performed with primers 1RNAF (5′-TACCTTTGACCAATCACGCT-3′) and CO11R (5′-GGTCAATGTTCAGAAATTTGTGG-3′) as previously described (2). A PCR product approximately 510 bp in length was obtained and sequenced at the Oklahoma State University Core Facility. Sequences were compared with those in NCBI's nucleotide database using BLAST and had 97% identity with two sequences from M. marylandi (KC473862.1 and KC473863.1) and the next most similar species being M. graminis (JN241898.1) with 83% identity. To our knowledge, this is the first report of the root-knot nematode M. marylandi in Oklahoma. As bermudagrass becomes more commonly used for putting greens in the turfgrass transition zone, M. marylandi may become a more common and damaging pathogen in the region. References: (1) A. A. Elmi et al. Grass For. Sci. 55:166, 2000. (2) M. A. McClure et al. Plant Dis. 96:635, 2012. (3) J. L. Starr et al. Nematrop. 37:43, 2007.

Abstract:

1032 Background: CC is the 2nd cause of cancer death in women in Argentine. Early detection following screening programs has demonstrated to reduce mortality in CC. The evaluation of the efficacy and efficiency of CC SP is associated with the proportion of positive cases in the smears and with the relation between pre-invasive lesion (CIN III) and invasive carcinoma (IC) in the biopsies done in positive cases. In 1997 a population based CC screening program was started in our province. The objective of this trial was to determinate the relationship between CIN III and IC in the program population and compare this results with this proportion in pre-program years an as an indicator of the quality of the program. Methods: We analyzed 2446 cervix biopsies performed in public Hospitals of Tucuman between 1985 and 2004 (archives of biopsies of the Serv. de Patología del Hosp. Centro de Salud Z. Santillán, Regional M. Belascuain and Hospital Angel C. Padilla), Since 1997 biopsies were done in patients within the SP We analyzed the proportions of pre-invasive lesions (CIN III) and invasive lesions in periods of 4 years. Results: The proportion of CIN III by periods of 4 years was: 85–88: 13%, 90–92: 20%; 93–96: 20%; 97–00 32% and 01–04 37%. The proportion of CIN III in pre-screening years was 21% and during the SP: 54%. Conclusion: These results shows an increase of CIN III over IC which could translate into a better survival. The quality control analysis of the public programs must be central in the public health policy. [Table: see text] No significant financial relationships to disclose.

Abstract:

AbstractEvidence on the association between maternal Hb concentration and preterm birth (PTB) risk is inconclusive. This paper aimed to explore whether women with anaemia or high Hb level before pregnancy would be at higher risk of PTB. We conducted a population-based cohort study with 2 722 274 women aged 20–49 years, who participated in National Free Pre-Pregnancy Checkups Project between 2013 and 2015 and delivered a singleton before 2016 in rural China. Logistic models were used to estimate OR and 95 % CI after adjusting for confounding variables. Restricted cubic spline models were applied to evaluate the dose–response relationships. A total of 192 819 (7·08 %) women had preterm deliveries. Compared with women with Hb of 110–149 g/l, the multivariable-adjusted OR for PTB was 1·19 (95 % CI 0·98, 1·44) for women with Hb<70 g/l, 1·01 (95 % CI 0·97, 1·03) for 70–99 g/l, 0·96 (95 % CI 0·95, 0·98) for 100–109 g/l, 1·04 (95 % CI 1·01, 1·06) for 150–159 g/l, 1·11 (95 % CI 1·05, 1·17) for 160–169 g/l and 1·19 (95 % CI 1·11, 1·27) for ≥170 g/l, respectively. The multivariable-adjusted OR for very PTB (VPTB) was 1·07 (95 % CI 1·03, 1·12) and 1·06 (95 % CI 1·01, 1·12) for women with Hb <110 and ≥150 g/l, compared with those with Hb of 110–149 g/l, respectively. Our study identified a U-shaped relationship between maternal preconception Hb concentration and PTB risk. Both preconception anaemia and high Hb level can significantly increase VPTB risk. Appropriate intervention for women with abnormal Hb levels before pregnancy is very necessary.

Abstract:

1510 Background: Irinotecan and temozolomide compare favorably to regimens tested in recurrent GBM. RTOG 04–20 intensifies the Stupp R, et al. (N Engl J Med. 2005 Mar 10;352(10):987–96) adjuvant regimen, using irinotecan and temozolomide in place of temozolomide alone. Methods: Adult patients with newly diagnosed histologically confirmed, supratentorial GBM were eligible. Subjects began temozolomide 75mg/m2 daily the night before initiation of EBRT, and continued until the final day of RT. Pneumocystis prophylaxis was begun prior to RT and for 2 weeks following RT. Within 6 wks after EBRT, subjects with stable or improved MRI were scheduled to receive temozolomide 150mg/m2 on days 1–5, and irinotecan 200mg/m2 on days 1 and 15 of 28 day cycles × 12. Clinical assessments and post contrast MRI are required prior to EBRT, after RT, and after every 2 treatment cycles. Only nonenzyme inducing anticonvulsants were allowed. Results: Accrual of 170 patients was completed in September 2005 with 140 patients currently evaluable. Median age is 57yr, 80% were RPA class III or IV. Prior to cycle 1 of adjuvant therapy, 32 subjects withdrew (20 progressed, 4 toxicity). Of the first 25 subjects receiving irinotecan, 10 suffered grade 3–4 hematologic toxicities in the initial 3 cycles. The protocol was modified by dose reducing irinotecan to 100mg/m2 in cycle 1, escalating to 150 and 200mg/2 in subsequent cycles only if no dose limiting hematologic toxicity occured. No data is yet available on patients who started adjuvant therapy at the lower dose of irinotecan. Diarrhea and constitutional symptoms are the most common nonhematologic toxicies Conclusions: Irinotecan 200mg/m2 days 1 and 15 with temozolomide 150mg/m2 days 1–5 of 28 day cycles was well tolerated treating recurrent GBM, but this regimen was too myelosuppressive when given after concomitant low dose temozolomide and EBRT. Primary outcome data should be available in late 2006. [Table: see text]

Abstract:

Abstract BACKGROUND It is challenging to discriminate radiation necrosis from tumor progression, especially in malignant glioma. Therefore many radiological imaging studies have been reported. In this study, we performed a systematic review of radiological diagnosis for radiation necrosis and analyzed the best radiological imaging for malignant glioma. METHODS We divided diagnostic approaches into two categories as follows-CT and MRI (conventional radiological imaging studies), and SPECT and PET (nuclear medicine studies). Our librarians conducted a comprehensive systematic search on Pub Med, Cochrane Library, and the Japan Medical Abstract Society up to March 2015. The searching keywords included radiation necrosis, recurrence, imaging modalities such as MRI, diagnosis, and differential. In a meta-analysis, diagnostic odds ratio (DOR) was calculated. A subanalysis was performed, dividing into tumor types, gliomas and metastatic brain tumors. RESULTS Of 188 and 239 records extracted from the database, 20 and 26 studies were included in the meta-analysis after exclusion of case reports and studies with incompatible content and insufficient information. Gd-enhanced MRI exhibited the lowest sensitivity (63%) and DOR (2.2). On the other hand, combined multiple imaging studies including MRS and perfusion image displayed the highest sensitivity (96%) and DOR (5.9). In the subanalysis for glioma, Gd-enhanced MRI and 18F-FDG-PET revealed low DORs (1.7 and 2.3). Conversely, 18F-FET-PET and combined multiple imaging studies showed high DORs (6.8 and 5.9). CONCLUSIONS Gd-enhanced MRI had low diagnostic ability for differentiation of radiation necrosis. In glioma patients, 18F-FDG-PET was not useful to discriminate radiation necrosis from tumor progression. Combined multiparametric imaging including lesional metabolism and blood flow could enhance diagnostic accuracy and be useful to differentiate radiation necrosis from tumor progression even in glioma patients.

Abstract:

The problem of burns is relevant in the world and in Ukraine. Thermal injury is not limited to local tissue changes, but causes significant morphofunctional disorders in the organs and systems of the affected organism, the result of this factor is a violation of blood supply to organs, including the pancreas. The aim of this work was to establish the histological condition of the vessels of the pancreas after experimental thermal injury. The experiments were performed on 40 adult white rats. Burns were applied under thiopental-sodium anesthesia with copper plates at a temperature of 96-97℃ on the epilated surface of the skin of the back of animals for 15 seconds (the lesion area was 18-20 % of the body surface). Decapitation of animals was performed on 1, 7, 14 and 21 days of the experiment. Pieces of the pancreas were taken for histological examination and processed according to generally accepted methods. Histological sections 5-6 μm thick were stained with hematoxylin-eosin, impregnated with silver by the Gordon-Sweets method, as well as staining by the tricolor method of MSB (ORB). Histological specimens were examined and documented using an SEO SCAN light microscope and a Vision CCD Camera. In the early stages of the experiment revealed a violation of the blood supply to the body. The lumens of most arteries were found to be dilated, and the wall thickness increased due to hypertrophy of the smooth muscle cells of the media. Leukocyte infiltration was determined in the perivascular spaces. The lumens of the small and middle veins were dilated and filled with blood, with parietal thrombi in some of them. Capillaries are characterized by a moderate expansion of their lumens, stasis. There was swelling of the paravasal spaces. Significant destructive-degenerative, inflammatory and sclerotic changes were observed in the later stages of the experiment. The walls of the vessels were moderately swollen. Smooth myocytes of the arterial media were hypertrophied, the cytoplasm was vacuolated, and contained pyknotically altered nuclei. Adventitia of vessels, especially veins, was swollen and fibrous. Occasionally there were violations of the wall of the components of the microcirculatory tract, which was accompanied by hemorrhage. There are single thrombosed vessels, spasmed arterioles, hemocapillaries with dilated lumens. The greatest changes, especially on the 21st day, were the adventitial membrane with the growth of collagen and reticular fibers. The blood supply to the vessels was moderate. Vessels of small diameter and hemomicrocirculatory tract were also significantly altered, their wall is deformed, thin, hom*ogeneous. The lumens are dilated, with the formation of blood clots. Thus, in the early stages of the experiment there are adaptive-compensatory processes and initial destructive changes in the walls of the vessels of the organ, manifested by stasis, thrombosis, edema, deformation, infiltration of the wall and perivascular space. In the late period, significant destructive-degenerative changes in the structure of the walls of the vessels of the pancreas, accompanied by hemorrhage, inflammation, sclerosis of the wall and edema of the adventitia. These changes lead to a deterioration of blood supply and, consequently, a decrease in the functional activity of the body.

Abstract:

Abstract Adolescence has been claimed since the seventies to be associated to a a bad prognosis in childhood ALL. Out of 4658 patients with ALL, 258 adolescents (15–20 year old)(5.5%) were treated in the successive FRALLE 83, FRALLE 87–89, FRALLE 92 (pilot phase), FRALLE 93 and FRALLE 2000 protocols. The main characteristics were: a sex ratio of 1.8 (M/F), a B-lineage in 71% of the cases vs T lineage in 29%, and a median WBC of 12 G/L (9–1000). Translocation and fusion transcripts were searched for in 120 evaluable BCP-ALL: t(9;22)/BCR-ABL, 8 pts (6%); t(1;19)/E2A-PBX1, 12 pts (10%); t(4;11)/MLL-AF4, 4 pts (3%). Out of 75 evaluable pts t(12;21)/TEL-AML1 was found in only 4 pts (3%). 242 out of 258 adolescents were in CR at the end of induction therapy(EOI)(94%) without any significant difference according to the era. Nevertheless a major difference in the 3y and 5y EFS was found: Number of pts CR at EOI (%) 3y EFS (%) 5y EFS (%) 10y EFS (%) *: p=.04; **: p=.04 Eighties (F83, F87–89) 100 93 42 +/− 5 35 +/− 5 35 +/− 5 Nineties (F92, F93) 84 93 71 +/−5* 67 +/− 5** 67 +/− 5 2000– (F2000) 74 96 86 +/−5* 86 +/− 10** NYA The main modification introduced in the nineties was the adoption of a double delayed intensification for the good early responders. Autologous BMT or allogenic BMT were indicated in bad early responders (D8 poor prednisone response, D21 marrow M3 response) and/or unfavourable cytogenetics. The better results of the 2000 protocol can mainly be explained by the further intensification of chemotherapy between induction and delayed Intensification 1 and before delayed Intensification 2. These better results were obtained despite decreasing the indications of BMT (6 performed vs 20 in the nineties) and of CNS irradiation (100% in the nineties vs 35% in the current era, including the TBI for BMTs). Conclusions:excellent results can now be achieved in adolescents with ALLthis study emphasizes again the need to treat adolescents with ALL according to pediatric intensive protocols and not “adult-type” protocols, as we recently suggested (Boissel et al, J Clin Oncol 2003).Whether this could also be applied to young adults remains to be demonstrated but seems appealing.

Abstract:

498 Background: PM from CRC are associated with poor outcomes; however, molecular differences are not defined. Methods: We compared tumor profiles of pCRC and PM patients (pts) from Caris Life Sciences. Testing included next-generation sequencing (NGS) of 592 genes, immunohistochemistry (IHC), copy number variants (CNV), microsatellite instability (MSI) and tumor mutational burden (TMB). Mutations were termed pathogenic (PATH) or variants of undetermined significance (VUS). TMB in mutations/Mb (MMB) was compared. Results: 617 pCRC and 348 PM pts had similar gender (55% male) and age (median 59). 232 pCRC were left-sided (LS), 189 right-sided (RS), 147 rectum (R) and 49 not otherwise specified (NOS); PM were 45 RS, 29 LS, 22 R and 252 NOS. For pts with IHC testing, expression was increased in PM in TOPO1 (62% v. 52%, p < 0.01), ERCC1 (27% v. 18%, p < 0.01) and MLH1 (96% v. 92%, p < 0.05) and decreased in PD-1 (36% v. 65%, p < 0.01), TOP2A (76% v. 100%, p < 0.01) and PTEN (64% v. 72%, p < 0.05). By sidedness, LS PM were more frequently TOP01 and PD-L1 and less commonly MGMT positive compared to pCRC. PTEN IHC was higher in R pCRC than PM. 7 CNVs were increased in PM ( ADGR2A2, CCND1, ELL, FGF3, FGF4, JAK3 and PDGFRB) and FLT3 CNVs were decreased. MYC CNVs were more common in RS PM compared to pCRC. No difference was seen in PM and pCRC PATHs in KRAS, BRAF, SMAD2, SMAD4, PTEN. PM had more PATHs in GNAS (8% v. 1%, p < 0.01) while pCRC PATHs were increased in APC (76% v. 48%, p < 0.01), TP53 (72% v. 53%, p < 0.01), ARID1A (29% v. 12%, p < 0.05), PIK3CA (22% v. 15%, p < 0.05) and FBXW7 (13% v. 7%, p < 0.01). LS PM had increased FLCN PATHs (12% v. 2%, p < 0.01); R PM had more PATHs in KMT2D (20% v. 1%, p < 0.01) and RNF43 (13% vs. 3%, p < 0.05). VUS were increased in 39/592 (7%) genes for PM compared to pCRC. No MSI or fusion difference was seen. 53% pCRC (median = 8) pts had TMB ≥8 MMB compared to 43% PMs (median = 7; p = 0.03); no TMB difference was seen for LS, RS or R subgroups. Conclusions: Compared to pCRC, PM had more PATHs in GNAS and less in classic CRC markers APC and TP53. While TMB was generally lower in PM, differences in IHC expression, CNV and VUSs may serve as biomarkers for PM requiring further study.

Abstract:

Background: Chronic kidney disease (CKD) is a clinically impaired kidney degradation syndrome, which commonly is diagnosed based on glomerulus filtration rate (GFR). Renal parenchymal resistive index and the renal cortex echogenicity are ultrasound parameters that have been reported correlate with GFR values. This study aims to determine the sensitivity, specificity, positive predictive value, and negative predictive value between renal intra-parenchymal resistive index and renal cortical echogenicitybased on GFR in CKD patients.Materials and Methods: This study is a cross sectional design. A renal ultrasound examination was performed to forty one CKD patients to assess the resistive index of the renal intra-parenchymal artery and the echogenicityof the renal cortex. The creatinine serum levels were obtained from the patients, as the gold standard of CKD diagnosis. Statistical data processing uses diagnostic test and Inter class Correlation Coefficients (ICC).Results: The sensitivity, specificity, positive predictive value, and negative predictive value of renal intraparenchymal resistive indexes were 23%, 79%, 33%, and 69% respectively. Sensitivity, specificity, positive predictive value, and negative predictive value between renal cortex echogenicity were 23%, 96%, 75%, and 73% respectively. The ICC analysisreported a Single Rater value of 0.1538 and Average of Raters 0.3528.Conclusion: Renal intra-parenchymal resistive artery was more specific than renal cortex echogenicity for diagnosing patients with chronic kidney disease.International Journal of Human and Health Sciences Vol. 04 No. 03 July’20 Page : 194-199

Abstract:

Purpose To study minimal disseminated disease (MDD) in children with Burkitt's lymphoma (BL) and to determine its impact on prognosis. Patients and Methods We established a simplified long-distance polymerase chain reaction (LD-PCR) assay that can amplify up to 15 to 20 Kb of DNA sequence, making it possible to detect the t(8;14) at the genomic level with sensitivity of 10−4. We prospectively studied diagnostic biopsies and bone marrow aspirates from 134 patients affected by BL. Results A specific LD-PCR product was detected in 96 (72%) of 134 BL biopsies. Among 84 patients with t(8;14) positivity on tumor biopsy and bone marrow (BM), 26 (31%) had LD-PCR–positive BM, and 15 (18%) were positive at standard morphologic analysis. Twenty (85%) of 26 MDD-positive patients belonged to the R4 risk group, according to Berlin-Frankfurt-Munster definition. The 3-year progression-free survival was 68% (SE, 10%) in MDD-positive patients in R4 compared with 93% (SE, 5%) in MDD-negative patients in R4 (P = .03). By multivariate analysis (including MDD, sex, lactate dehydrogenase, CNS involvement), only MDD was predictive of higher risk of failure (hazard ratio, 4.7; P = .04). Conclusion MDD identifies a poor-prognosis subgroup among children with high-risk BL. To improve disease control in these patients, a more effective risk-adapted therapy, possibly including anti-CD20 monoclonal antibody, should be considered.

Abstract:

ABSTRACTFour groups of six cows were fed from 12 weeks before calving to achieve condition scores at calving of 3·15 (F) and 2·15 (T). For the first 20 weeks of lactation all cows were given 8 kg concentrate (13·93 MJ metabolizable energy (ME) per kg dry matter (DM), 200 g crude protein (CP) per kg DM) which contained either a high or a low dietary concentration of undegradable dietary protein (UDP) (70 g/kg DM, H, and 49 g/kg DM, L) and 3 kg sugar-beet pulp. Hay was also offered ad libitum.There was no significant effect of treatment on milk yields over the first 20 weeks of lactation (overall mean yield 27·3 (s.e. 3·65) kg/day) but cows in group FL tended to produce less (P > 005). Milk composition was similar for all groups (48·8 (s.e. 5·12) g fat per kg, 27·2 (s.e. 1·48) g protein per kg and 49·0 (s.e. 2·52) g lactose per kg). DM intakes for groups FH, FL, TH and TL were 18·1, 16·9, 17·5 and 18·2 (s.e.d. 0·58) kg/day, respectively (TL v. FL, P < 0·05; FH v. FL, P < 0·05). Mean condition scores for groups FH, FL, TH and TL respectively, were 3·T7, 3·13, 2·21 and 2·08 (s.e.d. 0·18) at calving; 2·25, 2·50, 2·21 and 1·83 (s.e.d. 0·27) in week 10; and 2·46, 2·83, 2·46 and 1·96 (s.e.d. 0·35) in week 20, of lactation. Groups FH, FL, TH and TL lost proportionately 0·09, 0·02, 0·01 and 0·04 (s.e.d. 0·035) of their calving live weights, respectively, over 20 weeks of lactation.It is concluded that increasing the supply of UDP to cows which are fat at calving results in a greater negative energy balance and a small though non-significant increase in mobilization of body fat reserves. At the dietary ME and protein concentrations used, the thin cows could not respond to increased UDP supply and their intake was limited by physical restriction.

Abstract:

5054 Background: Patients with relapsed seminoma are treated with salvage chemotherapy. Current therapeutic options include cisplatin plus ifosfamide based regimens or high dose chemotherapy with PBSCT. At Indiana University HDCE was usually reserved for third line therapy for relapsed seminoma, unless there were visceral metastases or relapse within 3 months of initial chemotherapy. Methods: Retrospective review of 35 consecutive patients with pure seminoma (median 38 yrs; range 23–56 yrs) that received HDCE with PBSCT from 2/96–12/04. Cytoreductive chemotherapy with 0–2 courses of vinblastine, ifosfamide, and cisplatin (VeIP) preceeded HDCE. Carboplatin dosage was 700mg/m2 for 3 days plus etoposide 750mg/m2 for 3 days. A second course of therapy was given after hematopoietic recovery. Results: Treatment related mortality was seen only in patients that received two or more prior regimens (median age 45 yrs, range 27–56 yrs) and occurred in 4/35 (11%) patients. One man died of AML at 62 months (see table below). 26/35 (74%) are continuously NED (cNED). 15/35 patients received 1 prior regimen and 14/15 (93%) are cNED. 20/35 patients received =2 prior regimens and 12/20 (60%) are NED. 14/35 patients had prior XRT. 10/14 (71%) are cNED. 6/14 patients had prior XRT and =2 prior regimens and 3/6 (50%) are NED. Conclusions: HDCE with PBSCT has a high cure rate even when used as 3rd line or later therapy. Because of the 93% continuous NED rate when used as 2nd line therapy and the increased treatment related mortality when used as 3rd line therapy we now recommend HDCE as initial salvage therapy. [Table: see text] No significant financial relationships to disclose.

Abstract:

ABSTRAK Mengetahui pengaruh 3 komponen Intellectual Capital yang terdiri dari Value Added Capital Employed (VACA), Value Added Human Capital (VAHU), dan Structural Capital Value Added (STVA) dan mengetahui pengaruh Rate of Growth of Intellectual Capital (ROGIC)terhadap proporsi pembiayaan bagi hasil bank umum syariah di Indonesia periode 2015-2020 merupakan tujuan dari penelitian ini. Menggunakan regresi data panel serta data sekunder dengan mengumpulkan laporan tahunan bank umum syariah. Memanfaatkan Sampel 9 bank umum syariah yang terdaftar pada OJK dengan diseleksi menggunakan metode purposive sampling. Variabel Intellectual Capital diukur dengan metode Islamic Banking Value Added Intellectual Coefficient (IB-VAIC). Sedangkan untuk variabel proporsi pembiayaan bagi hasil menggunakan pengukuran profit sharing ratio. Hasil penelitian menunjukkan bahwa Value Added Capital Employed (VACA), Value Added Human Capital (VAHU), dan Structural Capital Value Added (STVA) dan Rate of Growth of Intellectual Capital (ROGIC) berpengaruh signifikan terhadap proporsi pembiayaan bagi hasil. Secara parsial, Value Added Capital Employed (VACA) berpengaruh signifikan negatif terhadap proporsi pembiayaan bagi hasil. Value Added Human Capital (VAHU) dan Structural Capital Value Added (STVA) memiliki pengaruh signifikan dengan arah positif pada proporsi pembiayaan bagi hasil. Rate of Growth of Intellectual Capital (ROGIC) tidak memiliki pengaruh pada proporsi pembiayaan bagi hasil. Kata Kunci: Intellectual Capital, VACA, VAHU, STVA, ROGIC, Proporsi Pembiayaan Bagi Hasil, Bank Umum Syariah. ABSTRACT Find out the effect of 3 components of Intellectual Capital, consisting of Value Added Capital Employed (VACA), Value Added Human Capital (VAHU), and Structural Capital Value Added (STVA), and find out the effect of the Rate of Growth of Intellectual Capital (ROGIC) on profit-loss sharing financing proportion of Islamic Banks in Indonesia from 2015-2020 is the goal of this research. Using panel data regression and secondary data by collecting the annual reports of Islamic Commercial Banks. Utilizing a sample of 9 Islamic Commercial Banks registered in OJK and selected using a purposive sampling method. The Intellectual Capital was measured by the Islamic Banking Value Added Intellectual Coefficient (IB-VAIC) method. Profit loss sharing financing proportion is measured by the profit sharing ratio. The results of this research showed that Value Added Capital Employed (VACA), Value Added Human Capital (VAHU), Structural Capital Value Added (STVA), and Rate of Growth of Intellectual Capital (ROGIC) had a significant effect on profit-sharing financing proportion. Partially, Value Added Capital Employed (VACA) has a negative significant effect on profit-loss sharing financing proportion. Value Added Human Capital (VAHU) and Structural Capital Value Added (STVA) have a positive significant effect on profit-loss sharing financing proportion. The rate of Growth of Intellectual Capital (ROGIC) does not affect the profit-sharing financing proportion. Keywords: Intellectual Capital, VACA, VAHU, STVA, ROGIC, Profit Loss Sharing Financing Proportion, Islamic Banks. DAFTAR PUSTAKA Aryee, S., Walumbwa, F. O., Seidu, E. Y. M., & Otaye, L. E. (2016). Developing and leveraging human capital resource to promote service quality: Testing a theory of performance. Journal of Management, 42(2), 480–499. https://doi.org/10.1177/0149206312471394 Ascarya. (2010). The lack of profit-and -loss sharing financing in Indonesia’s. Riebs, 1(1), 70–98. Ascarya, A., & Yumanita, D. (2007). Mencari solusi rendahnya pembiayaan bagi hasil di perbankan syariah Indonesia. Buletin Ekonomi Moneter dan Perbankan, 8(1), 7–43. https://doi.org/10.21098/bemp.v8i1.127 Bayraktaroglu, A. E., Calisir, F., & Baskak, M. (2019). Intellectual capital and firm performance: an extended VAIC model. Journal of Intellectual Capital, 20(3), 406–425. https://doi.org/10.1108/JIC-12-2017-0184 Bontis, N., William Chua Chong, K., & Richardson, S. (2000). Intellectual capital and business performance in Malaysian industries. Journal of Intellectual Capital, 1(1), 85–100. https://doi.org/10.1108/14691930010324188 Buallay, A., Cummings, R., & Hamdan, A. (2019). Intellectual capital efficiency and bank’s performance: A comparative study after the global financial crisis. 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